Lab Technician (Molecular Biology)

Location: Bay Area, United States. On-site role with at least two later-start, later-finish workdays per week to support time-sensitive laboratory workflows.

Company

Developing organ-scale reversible cryopreservation technology to preserve donated organs at subzero temperatures without ice formation and rewarm them uniformly for transplantation.

What you will do

• Express recombinant proteins in E. coli and yeast using established culture, induction, and harvesting protocols.
• Purify proteins through affinity chromatography, concentration, and buffer exchange.
• Evaluate protein concentration, yield, and purity using SDS-PAGE and protein concentration assays.
• Prepare and replenish culture media, buffers, stock solutions, and experimental reagents through accurate weighing, dilution, pH adjustment, and sterilization.
• After training, maintain mammalian cell cultures through thawing, media changes, passaging, counting, viability assessment, and cryopreservation.
• Track samples, reagents, proteins, and cell stocks while maintaining accurate production, quality-control, and maintenance records.

Requirements

• Bachelor’s degree in Biology, Biochemistry, Bioengineering, Molecular Biology, Chemistry, or a related field.
• At least two years of hands-on laboratory experience, including academic research, internships, or industry experience.
• Hands-on experience with molecular biology and protein purification.
• Familiarity with SDS-PAGE, protein concentration assays, accurate pipetting, solution preparation, and routine laboratory calculations.
• Familiarity with aseptic technique, laboratory safety, and contamination-control practices.
• Strong organization, attention to detail, documentation skills, protocol adherence, task coordination, and communication of deviations.

Nice to have

• Experience expressing recombinant proteins in both E. coli and yeast.
• Experience with chromatography systems, larger-scale protein production, and batch control.
• Experience with routine mammalian cell handling and maintenance.
• Strong protein-production experience combined with interest in learning mammalian cell techniques.

Culture & Benefits

• Medical, dental, and vision insurance coverage.
• Flexible time off and paid holidays.
• Competitive salary and equity package.
• 401(k), FSA, and commuter benefits.
• Daily lunch and dinner, snacks, a monthly learning stipend, and an on-site fitness center.
• Quarterly team social budget.