8 дней назад
Scientist, Cell Engineering (CRISPR)
Мэтч & Сопровод
Для мэтча с этой вакансией нужен Plus
Описание вакансии
Текст:
TL;DR
Scientist, Cell Engineering (CRISPR): Deriving, expanding, characterizing, and editing primary cell lines from diverse non-model mammalian organisms for downstream SCNT with an accent on primary cell culture, gene delivery, and cellular assays. Focus on optimizing cell engineering workflows, validating edited lines with flow cytometry and sequencing approaches, and supporting genome engineering milestones.
Location: Dallas, TX; onsite role. Relocation assistance is available.
Company
is a biotech startup developing genetic and cellular engineering approaches to help restore ecosystems and biodiversity.
What you will do
- Isolate, expand, maintain, and biobank primary cell cultures from diverse non-model animal tissues.
- Design and execute experiments to generate and characterize primary cells from non-model mammalian organisms.
- Develop functional cellular assays and assess experimental workflows using cellular assays and next-generation sequencing.
- Perform CRISPR gene editing and use chemical transfection, electroporation, and lipid nanoparticle delivery methods.
- Screen and validate edited cell lines for downstream somatic cell nuclear transfer (SCNT) in collaboration with genome engineers.
- Track project milestones, maintain laboratory records, and prepare reports and presentations.
Requirements
- PhD in biological sciences, stem cell biology, genetics, or a related field; candidates with a BS/MS and significant relevant experience may also be considered.
- At least 4 years of hands-on experience with mammalian cell culture systems.
- Expert knowledge of primary cell derivation, culture, propagation, and biobanking.
- Expert hands-on experience with CRISPR gene editing and multiple gene delivery methods.
- Experience with flow cytometry, immunostaining, confocal microscopy, and microdissection.
- Strong aseptic technique, analytical ability, organization, communication, and problem-solving skills.
Nice to have
- Experience with automated cell culture workflows.
- Previous experience with mESC editing pipelines.
Culture & Benefits
- Fast-paced, collaborative, growth-oriented startup environment.
- Medical, dental, and vision coverage.
- Paid time off, company holidays, flexible spending accounts, and a company-matched 401(k).
- Paid parental leave at 100% salary for up to 12 weeks.
- Education reimbursement and an inclusive workplace committed to diversity.
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