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18 часов назад

Scientist in Protein Technologies Center - Cell Engineering / Molecular Biology

86 320 - 154 960$
Формат работы
onsite
Тип работы
fulltime
Грейд
senior
Английский
b2
Страна
US
Вакансия из списка Hirify.GlobalВакансия из Hirify Global, списка международных tech-компаний
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Описание вакансии

Текст:
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TL;DR
Scientist in Protein Technologies Center - Cell Engineering / Molecular Biology (CRISPR, Molecular Cloning): Engineering and validating cell lines for gene knockouts, knock-ins, stable expression, protein tagging, and structural cell biology studies with an accent on genome editing, complex DNA constructs, and stable cell-line generation. Focus on designing saturation-mutagenesis assays, confirming edits and genomic stability, preparing NGS libraries, and supporting protein interaction, localization, and drug-discovery research.

Location: Memphis, Tennessee, United States

Salary: $86,320–$154,960 per year

Company

hirify.global conducts biomedical research and translates scientific discoveries into treatments for children.

What you will do

  • Engineer cell lines with CRISPR gene editing to generate and validate gene knockouts, knock-ins, and endogenous protein tags.
  • Generate stable cell lines using non-viral and viral delivery systems.
  • Develop stable target-overexpressing cell lines, pools, and clones.
  • Prepare NGS libraries and perform saturation mutagenesis and functional assays.
  • Support structural cell biology, protein interaction, localization, and drug-discovery studies.
  • Confirm editing success and genomic stability throughout clonal line generation.

Requirements

  • Onsite work in Memphis, Tennessee, United States.
  • Bachelor's degree in a relevant scientific field is required.
  • Eight years of relevant experience with a bachelor's degree, six years with a master's degree, or three years with a PhD.
  • Experience with CRISPR gene editing, stable expression platforms, cell-line engineering, and molecular biology.
  • Experience with molecular cloning methods and large or complex DNA constructs, including combinatorial libraries.
  • Experience with site-directed or saturation mutagenesis is required.

Nice to have

  • PhD with at least five years of postdoctoral experience in molecular biology, synthetic biology, or a related discipline.
  • Experience with In-Fusion, LIC, PIPE, Gateway, FX, overlap-extension PCR, Gibson assembly, BioBrick, or recombineering.

Culture & Benefits

  • Collaborative, bench-to-bedside research environment within a multidisciplinary Protein Technologies Center.
  • Medical and prescription drug, dental, and vision plans.
  • Retirement, wellness, paid time off, educational, and family-friendly benefits.
  • Medical premiums are covered for employees, with additional dependent coverage costs.

Hiring process

  • Include a cover letter with the application.

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