18 часов назад
Scientist in Protein Technologies Center - Cell Engineering / Molecular Biology
86 320 - 154 960$
Мэтч & Сопровод
Для мэтча с этой вакансией нужен Plus
Описание вакансии
Текст:
TL;DR
Scientist in Protein Technologies Center - Cell Engineering / Molecular Biology (CRISPR, Molecular Cloning): Engineering and validating cell lines for gene knockouts, knock-ins, stable expression, protein tagging, and structural cell biology studies with an accent on genome editing, complex DNA constructs, and stable cell-line generation. Focus on designing saturation-mutagenesis assays, confirming edits and genomic stability, preparing NGS libraries, and supporting protein interaction, localization, and drug-discovery research.
Location: Memphis, Tennessee, United States
Salary: $86,320–$154,960 per year
Company
conducts biomedical research and translates scientific discoveries into treatments for children.
What you will do
- Engineer cell lines with CRISPR gene editing to generate and validate gene knockouts, knock-ins, and endogenous protein tags.
- Generate stable cell lines using non-viral and viral delivery systems.
- Develop stable target-overexpressing cell lines, pools, and clones.
- Prepare NGS libraries and perform saturation mutagenesis and functional assays.
- Support structural cell biology, protein interaction, localization, and drug-discovery studies.
- Confirm editing success and genomic stability throughout clonal line generation.
Requirements
- Onsite work in Memphis, Tennessee, United States.
- Bachelor's degree in a relevant scientific field is required.
- Eight years of relevant experience with a bachelor's degree, six years with a master's degree, or three years with a PhD.
- Experience with CRISPR gene editing, stable expression platforms, cell-line engineering, and molecular biology.
- Experience with molecular cloning methods and large or complex DNA constructs, including combinatorial libraries.
- Experience with site-directed or saturation mutagenesis is required.
Nice to have
- PhD with at least five years of postdoctoral experience in molecular biology, synthetic biology, or a related discipline.
- Experience with In-Fusion, LIC, PIPE, Gateway, FX, overlap-extension PCR, Gibson assembly, BioBrick, or recombineering.
Culture & Benefits
- Collaborative, bench-to-bedside research environment within a multidisciplinary Protein Technologies Center.
- Medical and prescription drug, dental, and vision plans.
- Retirement, wellness, paid time off, educational, and family-friendly benefits.
- Medical premiums are covered for employees, with additional dependent coverage costs.
Hiring process
- Include a cover letter with the application.
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