Senior Scientist I, Cell Line Development (CHO)

Location: North Chicago, Illinois, United States; Conditions also list South San Francisco, California, United States.

Salary: $96,500–$183,500 per year.

Company

hirify.global develops innovative medicines and solutions across immunology, oncology, neuroscience, and aesthetics.

What you will do

• Develop next-generation CHO cell line platforms for recombinant antibodies and complex biologics.
• Design and test vector constructs and engineer host cells to improve stable-cell-line mAb titers.
• Execute transfection, clone-generation, screening, and clone-characterization campaigns.
• Apply molecular and cellular assays, including DNA copy-number, RNA expression, titer, flow cytometry, ddPCR, and RT-qPCR analysis.
• Operate and interpret data from Octet, ViCell, CloneSelect Imager, Beacon, and related CLD instrumentation.
• Prepare technical reports, present findings, and collaborate with cell culture, analytical, purification, and bioinformatics colleagues.

Requirements

• BS with 10+ years of relevant experience, MS with 8+ years, or PhD in molecular biology, cell biology, biochemistry, or a related discipline.
• Experience designing vector improvements to increase recombinant antibody expression in stable CHO cells.
• Strong understanding of mammalian protein expression, including genomic and epigenetic regulation, transcription, translation, mRNA stability, and protein secretion.
• Proficiency with Geneious Prime or similar software for DNA vector, primer, and probe design and sequence alignment.
• Experience with CRISPR/Cas or comparable tools for knocking genes in or out, plus proficiency in mammalian cell culture and CLD instrumentation.
• Strong scientific communication, data interpretation, multitasking, timeline management, and digital laboratory notebook documentation skills.

Nice to have

• Industry cell line development experience in a CMC or drug-substance environment.
• Experience developing CHO expression platforms for GMP manufacturing.
• Experience with flow cytometry, expression-engineering strategies, and next-generation sequencing for genomic DNA or RNA analysis.
• Programming and data-science skills in Python or R for analysis and visualization.

Culture & Benefits

• Collaborative work across cell culture, analytical, purification, and bioinformatics functions.
• Comprehensive benefits package including paid vacation, holidays, sick leave, and medical, dental, and vision insurance.
• 401(k) and eligibility for short-term incentive programs.
• Work performed under corporate standards for safety, materials handling, conduct, and good documentation practices.